Targeting Of Site-specific Non-LTR Retrotransposons: Role Of Amino-terminal Domains

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Targeting Of Site-specific Non-LTR Retrotransposons: Role Of Amino-terminal Domains

Show simple item record Shivram, Haridha en_US 2012-04-11T20:56:30Z 2012-04-11T20:56:30Z 2012-04-11 January 2011 en_US
dc.identifier.other DISS-11447 en_US
dc.description.abstract Restriction-like endonuclease (RLE) bearing non-LTR retrotransposons are site-specificelements that integrate into the genome through target primed reverse transcription (TPRT). RLE-bearing elements have been used as a model system for investigating non-LTR retrotransposon integration. R2 elements target a specific site in the 28S rDNA gene. We previously demonstrated that the two major sub-classes of R2 (R2-A and R2-D) target the R2 insertion site in an opposing manner with regard to the pairing of known DNA binding domains and bound sequences―indicating that the A- and D-clades represent independently derived modes of targeting that site. Elements have been discovered that group phylogenetically with R2 but do not target the canonical R2 site. Here we extend our earlier studies to show that a separate R2-A clade element, which targets a site other than the canonical R2 site, does so by using the amino-terminal zinc fingers and Myb motifs. We further extend our targeting studies beyond R2 clade elements by investigating the ability of the amino-terminal zinc fingers from the nematode NeSL-1 element to target its integration site (This work was done by Dillon Cawley). Our data are consistent with the use of an amino-terminal DNA binding domain as one of the major targeting determinants used by RLE-bearing non-LTR retrotransposons to secure a protein subunit near the insertion site. This amino-terminal DNA binding domain can undergo modifications, allowing the element to target novel sites. The binding orientation of the amino- terminal domain relative to the insertion site is quite variable. en_US
dc.description.sponsorship Christensen, Shawn en_US
dc.language.iso en en_US
dc.publisher Biology en_US
dc.title Targeting Of Site-specific Non-LTR Retrotransposons: Role Of Amino-terminal Domains en_US
dc.type M.S. en_US
dc.contributor.committeeChair Christensen, Shawn en_US Biology en_US Biology en_US University of Texas at Arlington en_US masters en_US M.S. en_US

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