Freezing-induced Microstructural Changes Of Collagen Scaffolds

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Freezing-induced Microstructural Changes Of Collagen Scaffolds

Show simple item record Miller, Jeffrey David en_US 2007-08-23T01:56:02Z 2007-08-23T01:56:02Z 2007-08-23T01:56:02Z December 2006 en_US
dc.identifier.other DISS-1615 en_US
dc.description.abstract Understanding the effects of freezing on the functional properties of tissues is critical to cryopreservation of native and artificial tissues, and prognosis after cryotherapies. Although investigations have been performed to correlate macroscopic properties such as mechanical properties with freezing parameters, the effects are typically tissue-type dependent and no physical understanding is available to explain these effects. Since the mechanical and optical properties of a tissue are based on the characteristics of its extracellular matrix (ECM), understanding and quantifying these changes could assist in predicting the response a tissue will have to freezing under different circumstances. In this study, the microstructural changes induced to a type I collagen tissue equivalent construct after freezing to different end temperatures were visualized using macroscopic observation, as well as optical microscopy and scanning electron microscopy. The changes were then quantified using image analysis. The study found that freezing results in a thinner and transparent gel at the macroscopic level. Optical microscopy of the frozen region portrayed apparent "wrinkles" in the matrix, and scanning electron microscopy illustrated a sparser, more disorganized scaffold of fibers, though the fibers appeared to be thicker. Image analysis results indicate that freezing causes a significant increase in matrix porosity and fiber diameter. en_US
dc.description.sponsorship Han, Bumsoo en_US
dc.language.iso EN en_US
dc.publisher Mechanical Engineering en_US
dc.title Freezing-induced Microstructural Changes Of Collagen Scaffolds en_US
dc.type M.S. en_US
dc.contributor.committeeChair Han, Bumsoo en_US Mechanical Engineering en_US Mechanical Engineering en_US University of Texas at Arlington en_US masters en_US M.S. en_US
dc.identifier.externalLinkDescription Link to Research Profiles

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