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Resolution And Localization In Single Molecule Microscopy

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Resolution And Localization In Single Molecule Microscopy

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dc.contributor.author Ram, Sripad en_US
dc.date.accessioned 2007-09-19T21:53:11Z
dc.date.available 2007-09-19T21:53:11Z
dc.date.issued 2007-09-19T21:53:11Z
dc.date.submitted August 2007 en_US
dc.identifier.other DISS-1765 en_US
dc.identifier.uri http://hdl.handle.net/10106/630
dc.description.abstract Rayleigh's criterion is extensively used in optical microscopy to determine the resolution of microscopes. Despite its widespread use, it is well known that this criterion is based on heuristic notions and can be surpassed in a regular optical microscope. The inadequacy of Rayleigh's criterion has necessitated a reassessment of the resolution limits of optical microscopes. The thesis proposes a new resolution criterion that overcomes the limitations of Rayleigh's criterion. The new result predicts that there is no resolution limit, but that the resolvability depends on the number of detected photons. Analytical tools are introduced to estimate the distance from microscopy images. By imaging fluorescently labeled DNA nano-rulers, it is shown that distances as small as 12 nm can be measured from experimental data with accuracy as predicted by the new resolution criterion. The new result is derived by adopting a stochastic framework and using the theory concerning the Fisher information matrix. This approach is generalized to a wide variety of estimation problems in optical microscopy by deriving expressions for the limits to the accuracy of the parameter estimates. As an application, the thesis addresses the location estimation problem. Analytical formulae are derived that provide a limit to the accuracy with which the location of a microscopic object can be determined. These results are illustrated by considering specific image profiles that describe the image of a single molecule. Another contribution of this thesis is the development of a new microscopy technique called multifocal plane microscopy for tracking single molecules/particles in 3D. An important property of this technique is its improved depth discrimination capability, which in turn enables accurate determination of the axial location of the particle especially when it is close to the plane of focus. en_US
dc.description.sponsorship Ober, Raimund en_US
dc.language.iso EN en_US
dc.publisher Biomedical Engineering en_US
dc.title Resolution And Localization In Single Molecule Microscopy en_US
dc.type Ph.D. en_US
dc.contributor.committeeChair Ober, Raimund en_US
dc.degree.department Biomedical Engineering en_US
dc.degree.discipline Biomedical Engineering en_US
dc.degree.grantor University of Texas at Arlington en_US
dc.degree.level doctoral en_US
dc.degree.name Ph.D. en_US

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