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Using Shewanella Baltica Ecotypes As A Model For Transcriptional Variation At The Population Level

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Using Shewanella Baltica Ecotypes As A Model For Transcriptional Variation At The Population Level

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dc.contributor.author Hambright, William Sealy en_US
dc.date.accessioned 2011-03-03T21:53:18Z
dc.date.available 2011-03-03T21:53:18Z
dc.date.issued 2011-03-03
dc.date.submitted January 2010 en_US
dc.identifier.other DISS-10891 en_US
dc.identifier.uri http://hdl.handle.net/10106/5521
dc.description.abstract Eukaryotic studies have shown considerable transcriptional variation among individuals of the same population. Owing to the cost of sequencing entire eukaryotic genomes, tested organisms were assumed to be genomically similar or even identical. We overcame this necessary assumption by using four sequenced strains of the bacterium Shewanella baltica, (OS155, OS185, OS195, OS223), as models to assess transcriptional variation and ecotype formation within a prokaryotic population. The strains were isolated from various depths throughout a water column of the Baltic Sea occupying different ecological niches characterized by various abiotic parameters. Although their genome sequences are strikingly similar, when grown in the laboratory under identical conditions, all strains exhibited significantly different growth rates suggesting global expressional variation. To confirm the findings, custom microarray slides containing probes representing all four of the sequenced genomes were hybridized with two strains at a time, in a two color manner, using a loop design. A one way ANOVA designated 415 core genes to be differentially expressed between the four strains at a stringent P value of 0.001. Furthermore, when analyzing common gene sequences shared among 32 other strains within the water column, Ecotype Simulation software consistently grouped all four model strains into discrete ecotypes. Transcriptional pattern variations such as the ones highlighted here may be used as indicators of short-term evolution emerging from the formation of bacterial ecotypes. en_US
dc.description.sponsorship Rodrigues, Jorge en_US
dc.language.iso en en_US
dc.publisher Biology en_US
dc.title Using Shewanella Baltica Ecotypes As A Model For Transcriptional Variation At The Population Level en_US
dc.type M.S. en_US
dc.contributor.committeeChair Rodrigues, Jorge en_US
dc.degree.department Biology en_US
dc.degree.discipline Biology en_US
dc.degree.grantor University of Texas at Arlington en_US
dc.degree.level masters en_US
dc.degree.name M.S. en_US

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