RESEARCH COMMONS
LIBRARY

Integration Mechanism Of The Site Specific Non-LTR Retrotransposon, R2Bm

ResearchCommons/Manakin Repository

Integration Mechanism Of The Site Specific Non-LTR Retrotransposon, R2Bm

Show full item record

Title: Integration Mechanism Of The Site Specific Non-LTR Retrotransposon, R2Bm
Author: Hull, Christi Lynn
Abstract: The focus of this thesis is the mechanistic study of non-LTR retrotransposon replication using the site specific transposon R2Bm. Non-LTR retrotransposons integrate into new chromosomal sites by copying their RNA back into DNA at the site of insertion using an exposed chromosomal 3' OH to prime cDNA synthesis.1 Although some of the early steps of an integration event have been detailed, how the element completes integration remains unknown.¹ ² ³ ⁴ ⁵ ⁶ Clarifying the last integration step, known as second strand synthesis, is a major goal of this research. In order to accomplish this, R2 RNA and protein substrates were generated and used in biochemical assays to explore the aspects of RNP formation and second strand synthesis.
URI: http://hdl.handle.net/10106/4945
Date: 2010-07-19
External Link: https://www.uta.edu/ra/real/editprofile.php?onlyview=1&pid=1699

Files in this item

Files Size Format View
Hull_uta_2502M_10681.pdf 20.56Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record

Browse

My Account

Statistics

About Us