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A Systems-Theory Approach to the Analysis of Multiexponential Fluorescence Decay

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A Systems-Theory Approach to the Analysis of Multiexponential Fluorescence Decay

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Title: A Systems-Theory Approach to the Analysis of Multiexponential Fluorescence Decay
Author: Ford, Corey C.; Eisenfeld, Jerome
Abstract: A mathematical model of the fluorescence decay experiment based on linear systems theory is presented. The model suggests an experimental technique which increases the probability of correctly determining the decay constants of a multicomponent system. Using moment methods for data analysis,accuracy is improved by combining information obtained from several discrete experiments. Examples are presented to show that the analysis of a three component system composed of known standards is improved as the number of experimental determinations is increased from 1 to 4. The discrete measurements are made by changing the excitation and emission wavelengths.
URI: http://hdl.handle.net/10106/2369
Date: 1978-08

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