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Synthesis And Characterisation Of Raman Nanotags For Molecular Detection

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Synthesis And Characterisation Of Raman Nanotags For Molecular Detection

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dc.contributor.author Nyagilo, James Otieno en_US
dc.date.accessioned 2009-09-16T18:20:33Z
dc.date.available 2009-09-16T18:20:33Z
dc.date.issued 2009-09-16T18:20:33Z
dc.date.submitted January 2008 en_US
dc.identifier.other DISS-10139 en_US
dc.identifier.uri http://hdl.handle.net/10106/1874
dc.description.abstract The realization that most diseases are preceded or accompanied by changes in the genetic and cellular level of an organism has increased with the need for imaging modalities, with the sensitivity and specificity, to quantitatively detect biomolecules at those levels. Specifically there has been a need for the development of a modality that has the ability to detect multiple biomolecules from a single sample in order to increase accuracy of molecular diagnosis and allow for early detection of terminal diseases. Surface enhanced Raman spectroscopy provides potential for such a modality. The distinct spectrum of each Raman active molecule serves as a molecular "fingerprint" enabling it to be clearly distinguished from other Raman spectra. Although Raman signals are fundamentally much weaker than signals produced by most other modalities, they can be enhanced by the use of electron rich metallic nanoparticles such as gold nanoparticles by 14 - 15 orders of magnitude. Additionally, gold's reported biocompatibility and its well documented use in dentistry and in the treatment of Rheumatoid Arthritis give it a unique advantage over other labeling materials. In this thesis, the synthesis and characterization of gold nanoparticles for optimum Surface Enhanced Raman Scattering within the optical window is described. Using these nanoparticles, five of nanotags were developed distinguished from each other by the different reporter molecules on them. These nanotags are shown to remain stable under harsh conditions and have a great potential for detection of multi-cellular expression in vivo and in vitro. en_US
dc.description.sponsorship Dave, Digant en_US
dc.language.iso EN en_US
dc.publisher Biomedical Engineering en_US
dc.title Synthesis And Characterisation Of Raman Nanotags For Molecular Detection en_US
dc.type M.S. en_US
dc.contributor.committeeChair Dave, Digant en_US
dc.degree.department Biomedical Engineering en_US
dc.degree.discipline Biomedical Engineering en_US
dc.degree.grantor University of Texas at Arlington en_US
dc.degree.level masters en_US
dc.degree.name M.S. en_US
dc.identifier.externalLink http://www.uta.edu/ra/real/editprofile.php?onlyview=1&pid=1180
dc.identifier.externalLinkDescription Link to Research Profiles

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